Facilities
Welcome to the Panasik Lab
Assistant Professor in Departments of
Biology and Chemistry
B.S.. University of Wisconsin - Madison
Ph.D. Pennsylvania State University
Post Doctoral Johns Hopkins University
npanasik@claflin.edu
JST #221, Claflin University
Chris, Lateisha, and Delisa analyzing
bioinfromatics data.
The student bay area is equipped for
8 students.
Microbiology Bay
We use this area for plating bacteria and working with and
preparing culture media. Sterile Technique is
a must in this area! Equipped with standard microbiology supplies,
pipettmen, re-pipetors, and a variety of sterile supplies, this area and
its counterpart on the opposing bench is where a majority of
microbiology takes place.
Media Prep Area
General purpose area for weighing
reagents and preparing media.
Diagnostics Station
A temperature controlled 6 sample UV-vis
spectrophotometer for sample analysis, enzyme kinetics, and spectral
measurements over a range of temperatures. Also equiped with a
cuirculating chilling waterbath for low temperature measurments. this
station also has a digital microscope for viewing mocrobial cells and
recording protein crystal growth.
Incubators
High temperature and low temperature
incubators - both shaking floor models and static bench models are
integral to doing high and low temperature selections during bacterial
growth.
FPLC
A Biocad 600 FPLC aids in the
purification of proteins.
Sequencing Facilities
An in-house DNA sequencer aids in
identification of new mutations in the DNA sequence after a successful
round of Directed Evolution. We get approximately 900 bases of sequence
per sequencing reaction.
Lamilar Flow Hood
For an extra sterile environment when
working with strains that contain no selectable antibiotic markers.
Confocal 3D Imaging Microscope
For an extra sterile environment when
working with strains that contain no selectable antibiotic markers.
FPLC
A Biocad 600 FPLC aids in the
purification of proteins.
Storage
Sterile supplies. Entrance in Dr.
Panasik's domain. Its fuzzy
French Press
Using high pressure, over 20,000 PSI,
a French Pressure Cell breaks open bacterial cells so their cellular
contents may be accessed without exposure to excessive heat.
Lateisha folding up for the say
Claflin is equipped with wireless
access.
Additional
facilities include -70, -30, and -4 C freezers, high temperature
and low temperature floor model shaking incubators, high temperature,
low temperature, and mesophilic temperature static incubators, FPLC,
4x6 cold room, fume hood, gel densitometer, and high speed Beckman JS2
floor model centrifuge. 3 Student computers and several ethernet ports
are available to students in the lab. The lab also contains two high
speed computers (8 GHz RAM, 2 dual core 3.1 GHz processors one for
computational ab initio monte carlo calculations of protein folding and
dynamics performed on a LINUX operating system and the other running
Windows Vista. Each is equipped with a high speed graphics card and
Cystal Eyes 3D viewing devices. The lab also has a 3D projector and 10
Crystal Eyes glasses for teaching, discussing, and analyzing 3D
structures of proteins in large groups
